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Custom Designer

Custom Designer is a tool that collects basic target information and provides a consumable order summary and a custom panel file for cytoprofiling runs. With Custom Designer, you can enable a full custom protein batch for your cytoprofiling runs.

Create a Custom Design for Cytoprofiling Runs​

  1. From the left navigation pane under Runs, select Custom Designer.
  2. From the top right of the Custom Designer page, select Start a Design.
  3. On the Design Information page, enter a unique name for your design and optionally provide a description of your design.
  4. Select Next.
  5. On the Protein page, select the protein spike-in type and enter up to 88 protein target names to spike-in to a cytoprofiling run.

    Make sure that you follow the provided instructions in the wizard for formatting protein names.

  6. Select Next.
  7. On the Summary page, review your design summary and confirm your design information and customizations.

    If you need to change your design information or customizations, then select Back, and update the appropriate page of the Custom Designer wizard.

  8. Select Publish Design. Or, select Save as Draft to save a draft of your design.

    Only published designs can be used in a planned run or manual run on the instrument.

Format Custom Protein Targets​

Up to 88 protein targets can be added to a run. Format custom protein targets as follows:

  • Protein names can include up to 64 letters (A-Z, a-z), numbers (0-9), dash, dot, parentheses, and underscores. Do not include spaces.
  • Protein names must be unique. To increase the counts per cell of a single protein target, append a suffix such as _1, _2 to the target name, e.g., RAF1_1, RAF1_2.
  • For manual entry, separate protein names with a new line, paste from Excel, or upload the protein names in a CSV file format.

The following list are examples of custom protein target names:

phos-ATM
Plk1
phosS32-cFos
phosS78-HSP27
Rb
cFOS
JNK123
phosS133-CREB
cMyc
phosS807-Rb
AKT1
CDK1
CDK4
phosT180-p38
phos-JNK123
HSP60
Smad2
phosTY-ERK12
TAK1
p27
phosS46-p53
Bcl2
Chk1
phosS317-Chk1
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