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Prepare a Cytoprofiling Manifest

When you prepare a cytoprofiling run manifest, start from a template, or create and populate an original CSV file.

Manifest Sections​

A cytoprofiling run manifest file includes the following sections. Each section starts with a bracketed section heading that is followed by a row of column headings. Section and column headings are case insensitive. All subsequent rows list metadata for the section.

  • Wells: Specifies well information for cytoprofiling primary analysis.
  • Run Values: Adds custom information for user annotations.

Start from a Template​

  1. Download a template.
  2. Open the template in Excel or a text editor.
  3. Edit the template as desired (for example, modify metadata or add columns)
  4. Save the run manifest as a CSV UTF-8 file.
    • Allowed characters in file name are are letters, numbers, dash, dot, parentheses, and underscore.
    • Do not include spaces in the file name.

Run Manifest Templates​

Downloadable TemplateContentsAVITI™ OS Version
Single Well Run Manifest Template
  • Minimal metadata (uses default settings)
  • Example wells
    		• v3.0.0 and later
    12-Well Run Manifest Template
  • Minimal metadata (uses default settings)
  • Example wells
    		• v3.0.0 and later
    12-Well Antibody Screening Kit Run Manifest Template
  • Minimal metadata (uses default settings)
  • Example wells
    		• v3.4.0 and later
    12-Well Direct In Sample Sequencing (DISS) with Target to Cell Assignment Run Manifest Template
  • Minimal metadata (uses default settings)
  • Example wells
    		• v3.4.0 and later
    48-Well Run Manifest Template
  • Minimal metadata (uses default settings)
  • Example wells
    		• v3.4.0 and later
    48-Well Antibody Screening Kit Run Manifest Template
  • Minimal metadata (uses default settings)
  • Example wells
    		• v3.4.0 and later
    Target Cell Assignment Manifest Templates
  • Minimal metadata (uses default settings)
  • Example wells
    		• v3.4.0 and later

    Create an Original File​

    1. Open a new file in Excel or another application that can save CSV files.
    2. Enter any of the following section headings and their column headings:
    [RunValues]
    KeyName, Value
    [Wells]
    WellLocation, WellLabel, CellType, CellDiameter
    1. If necessary, add metadata to the Run Values and Wells sections.
    2. Save the run manifest as a CSV UTF-8 file.
      • Allowed characters in file name are are letters, numbers, dash, dot, parentheses, and underscore.
      • Do not include spaces in the file name.
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