Prepare a Cytoprofiling Manifest

When preparing a cytoprofiling run manifest, start with a template or create and populate an original CSV file.

Manifest Sections

A cytoprofiling run manifest file includes the following sections. Each section starts with a bracketed section heading followed by a row of column headings. Section and column headings are case insensitive. All subsequent rows list metadata for the section.

• Wells: Specifies well information for cytoprofiling primary analysis.
• Run Values: Adds custom information for user annotations.

Start from a Template

1. Download a template.
2. Open the template in Excel or a text editor.
3. Edit the template as desired: e.g., modify metadata, add columns.
4. Save the run manifest as a CSV UTF-8 file.
   • Allowed characters in file name are are letters, numbers, dash, dot, parentheses, and underscore.
   • Do not include spaces in the file name.

Template Manifests

Downloadable Template	Contents	AVITI OS Version
Single Well Run Manifest Template	Minimal metadata (uses default settings) Example wells	v3.0.0 and later
12 Well Run Manifest Template	Minimal metadata (uses default settings) Example wells	v3.0.0 and later

Create an Original File

1. Open a new file in Excel or other application that can save CSV files.
2. Enter any of the following section headings and their column headings.

[RunValues]
KeyName, Value
[Wells]
WellLocation, WellLabel , CellType, CellDiameter

3. If necessary, add metadata to the Run Values and Wells sections.
4. Save the run manifest as a CSV UTF-8 file.
   • Allowed characters in file name are are letters, numbers, dash, dot, parentheses, and underscore.
   • Do not include spaces in the file name.