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Target Information

Target information defines the sequences and associated metadata for each target site, which enables cell assignment in Cells2Stats for Direct In-Sample Sequencing (DISS) cytoprofiling runs that complete cell assignment for at least one target.

Columns​

The Target Information section includes the TargetSiteName, TargetName, Gene, and Sequence columns. When the Target Information section is populated, the TargetSiteName, TargetSiteName and Sequence columns are required. Data input in the Target Information section must match the information that is provided in the associated Teton Atlas custom workflow design.

ColumnConstraintsDefinitionAllowed Values
TargetSiteNameRequiredThe name of the target site that is sequenced in the cytoprofiling run and is present in the associated Teton Atlas custom design1-64 case-sensitive alphanumeric characters, hyphens (-), and underscores (_)
TargetNameRequiredThe unique name of the target that is associated with the target site1-64 case-sensitive alphanumeric characters, hyphens (-), and underscores (_)
GeneOptionalThe gene name that is associated with the target1-64 case-sensitive alphanumeric characters, hyphens (-), and underscores (_)
SequenceRequiredThe unique nucleotide sequence that is associated with the targetDNA sequence (A, T, C, G only)
ControlTypeOptionalIdentifies a target as a control type and is used in CytoCanvas visualizationNontargetingControl, TargetingControl, or blank

Custom Target-Level Metadata​

Custom columns are an optional addition to the Samples section and specify additional key-value metadata for samples. Custom columns must meet the following requirements:

  • Each custom column name is unique and includes case-sensitive variations.
  • A custom column cannot have the same name as a required column.
  • When a sample name is repeated, each corresponding value in the custom column must match exactly.
  • The key character limit is 1–64. Valid characters include lower- and upper-case letters, numbers, hyphens, and underscores.
  • The value character limit is 0–255 ASCII characters.

Target Specification Examples​

The following examples show how to construct a manifest for specific use cases:

Define Cell Assignment Targets For Multiple Target Sites​

[TARGET INFORMATION]
TargetSiteName,TargetName,Gene,Sequence,ControlType
TargetName1,SFPQ-spacer,SFPQ1880,CCGTGATATCAGCAGGTAGA,NontargetingControl
TargetName2,ZNF2-spacer,ZNF2602307,GAAGCAATACCTCATTAAAC,TargetingControl
TargetName3,EGFR-full-length,EGFR,TTCGACTCACGCGCTTACC,
TargetName4,EGFR-wild-type,EGFR,GGTGCCGCATGACGAGCC

Add Control Types to Targets​

[TARGET INFORMATION]
TargetSiteName,TargetName,Gene,Sequence,PerterbationName
1_FirstTarget,SFPQ-spacer,SFPQ1880,CCGTGATATCAGCAGGTAGA,Knockout1
1_FirstTarget,ZNF2-spacer,ZNF2602307,GAAGCAATACCTCATTAAAC,Knockout1
2_SecondTarget,EGFR-full-length,EGFR,TTCGACTCACGCGCTTACC,Knockout2
2_SecondTarget,EGFR-wild-type,EGFR,GGTGCCGCATGACGAGCC,Knockout3

Define Custom Metadata Per Target​

[TARGET INFORMATION]
TargetSiteName,TargetName,Gene,Sequence,PerterbationName
1_FirstTarget,SFPQ-spacer,SFPQ1880,CCGTGATATCAGCAGGTAGA,Knockout1
1_FirstTarget,ZNF2-spacer,ZNF2602307,GAAGCAATACCTCATTAAAC,Knockout1
2_SecondTarget,EGFR-full-length,EGFR,TTCGACTCACGCGCTTACC,Knockout2
2_SecondTarget,EGFR-wild-type,EGFR,GGTGCCGCATGACGAGCC,Knockout3
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