Prepare a Target Cell Assignment Manifest

When you prepare a target cell assignment manifest, start with a template or create an original CSV file.

Manifest Sections

A target cell assignment manifest file includes the following sections. Each section starts with a bracketed section heading that is followed by a row of column headings. Section and column headings are case-insensitive. All subsequent rows list metadata for the section.

• Target Site Settings: Define settings per target sites.
• Target Information: Adds target sequence information that includes target site to target mapping and unique target sequences for guide RNA applications.

Start from a Template

1. Download a template.
2. Open the template in Excel or a text editor.
3. Edit the template as desired (for example, modify metadata or add columns).
4. Save the run manifest as a CSV UTF-8 file CsvFileName/.

Template Manifests

Downloadable Template	Contents	AVITI OS Version
Single-Target Cell Assignment Manifest	Minimal metadata (uses default settings) Targets examples for a single target site	v3.4.0 and later
Two-Target Cell Assignment Manifest	Settings applied Targets examples for a multiple target site	v3.4.0 and later

Create an Original File

1. Open a new file in Excel. Or, open an application that can save .csv files.

2. Enter any of the following section headings and their column headings:

   [TARGET SITE SETTINGS]
   TargetSiteName,SettingName,Value,
   [TARGET INFORMATION]
   TargetSiteName,TargetName,Gene,Sequence

3. Add metadata to the Target Site Settings and Target Information sections.

4. Save the target cell assignment manifest as a CSV UTF-8 file CsvFileName/.